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Field
Current Value
Updated change
Name
NIR Rac1 Biosensor
Full name
Readout Method
FRET
Select Type ...
FRET
Intensity
FLIM
FLIM-FRET
Translocation
Excitation Ratiometric
Emission Ratiometric
BRET
Localization
Bioluminescence
FLINC
Ratiometric
Pubmed ID
29686359
Source Year
2018
Source Journal
Nat Chem Biol
Source Author
Shcherbakova DM, Cox Cammer N, Huisman TM, Verkhusha VV, Hodgson L
Other Sources
Addgene number
Components
miRFP720 | PAK1 p21-binding domain (PBD) | GSGGPPGSGGSG Linker | p21-binding domain (PBD) (H83D, H86D) | 58 AA linker | miRFP670 | 10 AA linker | Rac1
Sensing Element
PAK1 p21-binding domain | Rac1
Fluorescent Proteins
miRFP720 | miRFP670
Targeting
Unimolecular?
Unimolecular
Bimolecular or other
BS Family