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Field	Current Value	Updated change
Name	GACh2.0
Full name	GPCR-based acetylcholine sensor
Readout Method	Intensity	Select Type ... FRET Intensity FLIM FLIM-FRET Translocation Excitation Ratiometric Emission Ratiometric BRET Localization Bioluminescence FLINC Ratiometric
Pubmed ID	29985477
Source Year	2018
Source Journal	Nat Biotechnol
Source Author	Jing M, Zhang P, Wang G, Feng J, Mesik L, Zeng J, Jiang H, Wang S, Looby JC, Guagliardo NA, Langma LW, Lu J, Zuo Y, Talmage DA, Role LW, Barrett PQ, Zhang LI, Luo M, Song Y, Zhu JJ, Li Y
Other Sources
Addgene number	106073
Components	MR Trans membrane domains 1-5 |GG Linker | cpGFP| APSVA Linker |MR TM domains 6-7
Sensing Element	Muscarinic acetylcholine receptor (MR)
Fluorescent Proteins	cpEGFP
Targeting
Unimolecular?	Unimolecular Bimolecular or other
BS Family	[] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [2OG] - mOGsor [3CLPro] - Flip-GFP [3prime IP] - GFP AKT domains [3prime IP] - Homo FRET mCherry-Akt-PH [3prime IP] - InPAkt [5-HT] - 5-HT 3A CNiFER [5-HT] - dLight [5-HT] - G-GESS [5-HT] - GRAB-5HT [5-HT] - iSnFR [A1R] - A1R SPASM [Abl] - Abl Indicator [ACh] - ACh CNiFER [ACh] - ACh-Snifit [ACh] - GACh [ADP] - ADPrime [Akt] - Akind [Akt] - Akt translocation Reporters [Akt] - AktAR [Akt] - AktUS [Akt] - BKAR [Akt] - Dual Labeled Akt [Akt] - Eevee-Akt [Akt] - ReAktion [alpha2A-AR] - Receptor Conformation Sensor [Ammonium] - AmTrac [AMPK] - AMPfret [AMPK] - AMPKAR [AMPK] - ExRai-AMPKAR [Annexin 4] - NEX4 [Arabinose] - FLIPara [Arg] - FRET Arg Reporter [As3+] - SenALiB [aspartate] - iSnFR [Atg4a] - FRET-LC3B [Atg4b] - FRET-GATE-16 [ATM kinase] - ATOMIC [ATP] - ATeam [ATP] - EAF-based ATP biosensor [ATP] - iATPSnFR [ATP] - MaLion [ATP] - Nano-lantern ATP [ATP] - QUEEN [ATP] - Syn-ATP [ATP:ADP Ratio] - Perceval [Aurora B kinase] - Aurora B sensor [Aurora Kinase A] - AURKA Biosensor [B-Raf] - Prin-BRaf [Bax] - Bax Translocation reporter [BCAA] - OLIVe [Bcr-Abl] - Pickles [BDNF] - Bescell [beta Arrestin 2] - Beta Arrestin 2 ubiquitination biosensor [Beta1-AR] - dLight [Beta2-AR] - BRET^2 β2-AR Activation Probes [Beta2-AR] - dLight [Beta2-AR] - β2-AR SPASM [Beta2-AR] - Nb80 [Beta2-AR] - Receptor Conformation Sensor [C-Raf] - Prin-CRaf [Ca2+] - Ca-FLITS [Ca2+] - Ca2+ Snapshot [Ca2+] - CaMBI [Ca2+] - Cameleons [Ca2+] - Camgaroo [Ca2+] - CaMPARI [Ca2+] - CASE [Ca2+] - CatchER [Ca2+] - ddFP Ca2+ sensor [Ca2+] - ER-GCaMP [Ca2+] - ER-GECO [Ca2+] - FGCaMP [Ca2+] - FPX Ca2+ Sensors [Ca2+] - GAF-CaMP [Ca2+] - GAP [Ca2+] - GCaMP [Ca2+] - GECO [Ca2+] - GreenT-EC [Ca2+] - Nano-lantern Ca2+ [Ca2+] - NCaMP [Ca2+] - NTnC [Ca2+] - pericam [Ca2+] - RCaMP [Ca2+] - TN Ca2+ Sensors [CaM] - BSCaM [CaM] - MLCK-FIP [CaMKII] - Camui [CaMKII] - FRESCA [CaMKII] - K2alpha [cAMP] - cADDis [cAMP] - camps [cAMP] - CAMYEL [cAMP] - CUTie [cAMP] - Epac-S [cAMP] - Epac1 (delta DEP-CD) based cAMP biosensors [cAMP] - Flamindo [cAMP] - FPX [cAMP] - ICUE [cAMP] - mCRIS based cAMP biosensor [cAMP] - mICNBD [cAMP] - mICNBD-FRET [cAMP] - Nano-lantern cAMP [cAMP] - R-FlincA [cAMP] - R1alpha #7 [cAMP] - Split Luc cAMP biosensor [cAMP] - YFP-PKAc + CFP-PKAr [CaN] - CaN Activation [CaN] - CaNAR [CaN] - CaNARi [CaN] - DuoCaN [Caspase1] - FPX caspase 1 sensor [Caspase1] - SCAT1 [Caspase3] - CASPAM [Caspase3] - ddFP based [Caspase3] - DEVD [Caspase3] - DEVK [Caspase3] - EC-RP [Caspase3] - Far-Red Caspase sensors [Caspase3] - Flip-GFP [Caspase3] - Intein Based [Caspase3] - iProtease [Caspase3] - SCAT3 [Caspase3] - yDMQDc [Caspase8] - Bid cleavage sensor [Caspase8] - FPX caspase 8 sensor [Caspase8] - IC-RP [Caspase9] - SCAT9 [Cd2+] - Cd-FRET [Cdc42] - (CDC42) GEF Sensor [Cdc42] - CDC42 Biosensor [Cdc42] - Cdc42 FRET [Cdc42] - CDC42 Raichu [Cdc42] - Cdc42-2G [Cdc42] - ddFP Cdc42 Biosensor [Cdc42] - GDI Cdc42 FLARE [CDK1] - CyclinB1-Cdk1 activity sensor [CDK2] - CDK2 Translocation Reporter [Cell Cycle Phases] - Fucci [cGMP] - cGES [cGMP] - cGKI based cGMP sensors [cGMP] - cGull [cGMP] - CGY [cGMP] - CUTie [cGMP] - cyGNAL [cGMP] - cygnet [cGMP] - FlincG [cGMP] - PfPKG [Citrate] - FLIP-Cit [CO] - COSer [CRAC] - PH(crac)-GFP [CREB] - ICAP [Cu+] - CreiLOV [Cu+] - Yeast Copper regulator based [DAG] - Cys1-GFP [DAG] - Daglas [DAG] - DAGR [DAG] - Digda [DAPK1] - DAPK1 sensor [Dictyostelium GPCR] - Labeled G proteins [DNA methylation] - DNA methylation BiAD [Dopamine] - dLight : Dopamine [Dopamine] - Dopamine CNiFER [Dopamine] - GRAB-DA [DRD2] - iTango [eCB] - GRAB-eCB [EGFR] - ECaus [EGFR] - EGFR Reporter [EGFR] - FLAME [EGFR] - Picchu-Z [Enterokinase] - BRET-based [ERK] - EAS [ERK] - EKAR [ERK] - ERK-KTR [ERK] - Erkus [ERK] - ERKy [ERK] - FIRE [ERK] - FLINC-EKAR1 [ERK] - FPX ERK [ERK] - Miu2 [F-1,6-BP] - fructose‐1,6‐bisphosphate [FAK] - FAK Activation Biosensor [FAK] - FAK Autophosphorylation Biosensor [FAK] - FAK sensor [Fus3] - Far1-SKARS [Fyn] - FRET [Fyn] - saFRET [FZD4] - Receptor Conformation Sensor [FZD5] - Receptor Conformation Sensor [FZD6] - Receptor Conformation Sensor [FZD7] - Receptor Conformation Sensor [G1 Phase] - G1 Phase Biosensor [GABA] - GABA-Snifit [GABA] - iGABASnFR [GCK] - GCK Activation Biosensors [Gα] - Galpha activation [Gαi] - Gαi Sensor [Gαq] - Gαq Sensor [Gαs] - Gαs Sensor [Gαs] - Gs Activation BRET Assay [Gαs] - Nb37 based [Gln] - FLIPQ [Glucose] - FLIPglu [Glucose] - GGBP [Glucose] - GIP [Glucose] - Glifon [Glutamate] - FLIP Glt [Glutamate] - FLIPE [Glutamate] - GluSnFR [Glutamate] - iGluSnFR [Glutamate] - QBP based Gln Sensor [Glutamate] - Snifit-iGluR5 [Glycine] - glycine FRET sensor [GTP] - GEVAL [H2O2] - HyPer [H2O2] - roGFP based H2O2 sensor [H2O2] - Unnatural Amino Acid H2O2 sensor [H2O2] - Yap1 based H2O2 sensor [H2S] - pAzF H2S sensors [H3-S10p] - H3S10p biosensor [H3-S28p] - Histone Phosphorylation reporter [H3K9me3] - H3K9me3 BiAD [H3R] - Receptor Conformation Sensor [Halide Ions] - Clomeleon [Halide Ions] - Dual pH and Cl sensor [Halide Ions] - Halide Sensitive Fluorescent Proteins [Heme] - CHY [Heme] - CISDY [Heme] - Fluorescence quenching Heme [Heterotrimeric G Proteins] - αβγ trimerization [Hg(II)] - eGFP based Hg sensor [Hg(II)] - IFP based Hg sensor [His] - FHisJ [His] - FLIP-HisJ [Histone Acetylation] - Histac [Ins(1,3,4,5)P4] - Btk PH domain based [INSR] - Insulin Receptor Activation BRET assay [INSR] - Phocus [INSR] - sinphos [Insulin] - RINS [IP3] - FIRE [IP3] - fretino [IP3] - GFP-PH [IP3] - IRIS [IP3] - LIBRA [α1-AR] - α1-AR SPASM [α2A-AR] - dLight [α2A-AR] - α2-AR SPASM [α2A-AR] - α2A-AR + labeled G protein [α2A-AR] - α2A-AR activation sensor [JNK] - dJUN-FRET [JNK] - JNK-KTR [JNK] - JNKAR [JNK] - JuCKY [K+] - GEPII [K+] - GINKO [K27H3 methlytransferase] - K27 Reporter [K9H3 methyltransferase] - K9 Reporter [kappa-opioid receptor] - dLight [Kss1] - Ste7-SKARS [Lactate] - Laconic [LATS] - LATS-BS [LCACoAs] - LACSer sensor [Lck] - Lck Activation sensor [Ln3+] - LaMP [M1R] - M1R Activation Sensor [M3R] - Receptor Conformation Sensor [malonyl-CoA] - FapR-NLuc [maltose] - FLIPmal [maltose] - MBP [MAPK or MK2] - GMB [MARK] - MARK sensor [Matriptase] - ddFP-based [Mechanical Strain] - HP35 [Mechanical Strain] - PriSSM [Mechanical Strain] - stFRET [Mechanical Strain] - TSMod [Mg2+] - MagFRET [Mg2+] - MagIC [MLKL] - SMART [Mn2+] - MnLaMP [Molecular crowding] - CrGE [Molecular crowding] - Crowding sensor [Mpk1] - Mkk2-SKARS [Mpro] - BRET-based [Mpro] - Flip-GFP [MT1-MMP] - MT1-MMP Biosensor [MT2] - dLight [mTORC1] - TORCAR [mu-opioid receptor] - dLight [N-WASP] - N-WASP BS [NAD+] - NAD+ Biosensor [NAD+] - NAD-Snifit [NAD+/NADH Ratio] - Peredox [NAD+/NADH Ratio] - SoNar [NAD+/NADH Ratio] - T-Rex Based [NADH] - Frex [NADP+] - Apollo-NADP+ [NADP+] - NADPsor [NADP+:NADPH Ratio] - NADP-Snifit [NADPH] - iNap [NADPH] - Semi-synthetic BRET Sensor [NE] - NE CNiFER [Nicotine] - iNicSnFR [NO] - FRET-MT [NO] - geNOps [NO] - sGC based NO biosensor [O2] - dUnOHR hypoxia-reoxygenation sensor [O2] - FluBO [ODR-10] - OGOR [OGT] - O-GlcNAc Sensor [OHP] - OHSer [ONOO-] - pnGFP [opsin] - opsin SPASM [Orexin Neuropeptide] - dLight : Orexin [Oxytocin] - GRAB-OT [p38] - p38 activity reporter [p38] - p38-KTR [PA] - Pii [PAK1] - Pakabi [PDGFR] - PDGFR Biosensor [PDK1] - PARE [pH] - deGFP [pH] - Dendra [pH] - Dual pH and Cl sensor [pH] - E^xGFP [pH] - FluBpH [pH] - mtAlpHi [pH] - Native FP pH Sensitivity [pH] - pHaROS [pH] - pHCEC [pH] - pHluorins [pH] - pHRed [pH] - pHTomato [pH] - pHuji [pH] - pHusion [pH] - SRpHi [pH] - SypHer [pH] - XFpH [Phosphate] - FLIPPi [PI(3)P] - GFP-FYVE [PI(3)P] - GFP-PX [PI(3,4)P2] - cPHx3 [PI(3,4)P2] - Pippi-PI(3,4)P2 [PI(3,5)P2] - GFP-MLN1 [PI(4)P] - BRET PI(4)P sensors [PI(4)P] - Pippi [PI(4,5)P2] - CAY [PI(4,5)P2] - CYPHR [PI(4,5)P2] - ENTH-GFP [PI(4,5)P2] - FP-Tubby [PI(4,5)P2] - FPX PIP2 sensor [PI(4,5)P2] - PH(PLCdelta) [PI(4,5)P2] - Pippi-PI(4,5)P2 [PI(4,5)P2] - R-PRE [PIP3] - FLLIP [PIP3] - InPAkt [PIP3] - Labeled PH domains [PIP3] - PIP3 BRET sensor [PKA] - AKAR [PKA] - ART [PKA] - FLINC-AKAR1 [PKA] - PKA-KTR [PKA] - RLuc-PCA PKA [PKA] - Single Color PKA sensor [PKC] - CKAR [PKC] - Eevee-PKC [PKC] - IDOCKS [PKC] - ITRACK [PKC] - KCP [PKD] - DKAR [PKD] - G-PKDrep [PKM2] - PKAR [PMCA] - PMCA Activity Sensor [Pn] - EcPhnD based [PS] - 2xPH(EVECTIN2) [PS] - cPLA2-C2 [PS] - Lact-C2 [PS] - PKC-C2 [PS] - PLCdelta1-C2 [PTEN] - Rluc-PTEN-YFP [PTH1R] - Receptor Conformation Sensor [PTHR] - PTHR activation sensor [Pyruvate] - PdhR Based [Rab13] - Rab13 [Rab5] - Rab5 Raichu [Rac1] - ddFP Rac1 sensors [Rac1] - FLAIR [Rac1] - FLARE [Rac1] - GDI Rac1 FLARE [Rac1] - Rac1 Raichu [Rac1] - Single Chain Rac1 biosensors [Rac2] - Single Chain Rac2 biosensors [Rac3] - Single Chain Rac3 biosensors [Ral] - Raichu-Ral [Ran] - Ran FRET probes [Rap1 ] - Rap1 Raichu [Rap1 ] - Rap1-FLARE [Ras] - ddFP Ras Biosensor [Ras] - DORA Ras [Ras] - FRas [Ras] - Ras Raichu [Ras] - RasAR [redox status] - HSP33 [redox status] - redox-sensitive linker [redox status] - roFP [redox status] - rxRFP [redox status] - rxYFP [Rho] - GBD [RhoA] - GDI-RhoA FLARE [RhoA] - RhoA FLARE [RhoA] - RhoA FRET [RhoA] - RhoA Raichu [RhoB] - DORA RhoB [RhoC] - DORA RhoC [RhoC] - RhoC FLARE [RhoQ] - TC10 Raichu [ribose] - FLIPrib [ROCK] - Eevee-ROCK [ROS] - pHaROS [ROS] - pHaROS [RRas] - RRas Raichu [RSK] - Eevee-RSK [RTK] - Picchu [S Phase] - S Phase Biosensor [S6K] - Eevee-S6K [SAP3K] - SAP3K [SnRK2.6/OST1] - SNACS [Src] - Src Indicator [Src] - Srcus [sterol] - mCherry-D4H [sucrose] - FLIPsuc [superoxide] - mt-cpYFP [Temperature] - ELP [Temperature] - gTEMP [Temperature] - mRFP [Temperature] - tsGFP [TEV] - Flip-GFP [Trehalose] - FLIP [TrkB] - TrkB-PLC interaction [Trp] - FLIPW [Trp] - GRIT [Ubiquitination] - REACh-Ubiquitin [V+] - ArcLight Species Variants [V+] - Ci-VSP based sensors [V+] - Danio VSP based sensor [V+] - Kv Based V sensors [V+] - Na Channel based V Sensors [V+] - Nematostella VSP based sensors [V+] - rhodopsin based V sensors [VEGF] - BRET VEGF biosensor [ZAP-70] - ROZA [ZAP-70] - saFRET [Zn2+] - Atox1 WD4 Based Zn2+ Probes [Zn2+] - GZnP [Zn2+] - Minimal Zinc Finger Based Zn Probes [Zn2+] - Zap1 Based Zn Probes [Zn2+] - ZinCh
Notes

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