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Field	Current Value	Updated change
Name	EKAR
Full name	Extracellular signal-regulated kinase activity reporter
Readout Method	FRET	Select Type ... FRET Intensity FLIM FLIM-FRET Translocation Excitation Ratiometric Emission Ratiometric BRET Localization Bioluminescence FLINC Ratiometric
Pubmed ID	19033456
Source Year	2008
Source Journal	Proc Natl Acad Sci U S A
Source Author	Harvey CD, Ehrhardt AG, Cellurale C, Zhong H, Yasuda R, Davis RJ, Svoboda K
Other Sources
Addgene number
Components	mRFP1 | WW domain | 72 Gly Linker | ERK substrate (CdC25c) | ERK docking domain | EGFP
Sensing Element	WW domain | 7ERK substrate (CdC25c)
Fluorescent Proteins	mRFP1 | EGFP
Targeting	Cytosolic
Unimolecular?	Unimolecular Bimolecular or other
BS Family	[] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [] - [2OG] - mOGsor [3CLPro] - Flip-GFP [3prime IP] - GFP AKT domains [3prime IP] - Homo FRET mCherry-Akt-PH [3prime IP] - InPAkt [5-HT] - 5-HT 3A CNiFER [5-HT] - dLight [5-HT] - G-GESS [5-HT] - GRAB-5HT [5-HT] - iSnFR [A1R] - A1R SPASM [Abl] - Abl Indicator [ACh] - ACh CNiFER [ACh] - ACh-Snifit [ACh] - GACh [ADP] - ADPrime [Akt] - Akind [Akt] - Akt translocation Reporters [Akt] - AktAR [Akt] - AktUS [Akt] - BKAR [Akt] - Dual Labeled Akt [Akt] - Eevee-Akt [Akt] - ReAktion [alpha2A-AR] - Receptor Conformation Sensor [Ammonium] - AmTrac [AMPK] - AMPfret [AMPK] - AMPKAR [AMPK] - ExRai-AMPKAR [Annexin 4] - NEX4 [Arabinose] - FLIPara [Arg] - FRET Arg Reporter [As3+] - SenALiB [aspartate] - iSnFR [Atg4a] - FRET-LC3B [Atg4b] - FRET-GATE-16 [ATM kinase] - ATOMIC [ATP] - ATeam [ATP] - EAF-based ATP biosensor [ATP] - iATPSnFR [ATP] - MaLion [ATP] - Nano-lantern ATP [ATP] - QUEEN [ATP] - Syn-ATP [ATP:ADP Ratio] - Perceval [Aurora B kinase] - Aurora B sensor [Aurora Kinase A] - AURKA Biosensor [B-Raf] - Prin-BRaf [Bax] - Bax Translocation reporter [BCAA] - OLIVe [Bcr-Abl] - Pickles [BDNF] - Bescell [beta Arrestin 2] - Beta Arrestin 2 ubiquitination biosensor [Beta1-AR] - dLight [Beta2-AR] - BRET^2 β2-AR Activation Probes [Beta2-AR] - dLight [Beta2-AR] - β2-AR SPASM [Beta2-AR] - Nb80 [Beta2-AR] - Receptor Conformation Sensor [C-Raf] - Prin-CRaf [Ca2+] - Ca-FLITS [Ca2+] - Ca2+ Snapshot [Ca2+] - CaMBI [Ca2+] - Cameleons [Ca2+] - Camgaroo [Ca2+] - CaMPARI [Ca2+] - CASE [Ca2+] - CatchER [Ca2+] - ddFP Ca2+ sensor [Ca2+] - ER-GCaMP [Ca2+] - ER-GECO [Ca2+] - FGCaMP [Ca2+] - FPX Ca2+ Sensors [Ca2+] - GAF-CaMP [Ca2+] - GAP [Ca2+] - GCaMP [Ca2+] - GECO [Ca2+] - GreenT-EC [Ca2+] - Nano-lantern Ca2+ [Ca2+] - NCaMP [Ca2+] - NTnC [Ca2+] - pericam [Ca2+] - RCaMP [Ca2+] - TN Ca2+ Sensors [CaM] - BSCaM [CaM] - MLCK-FIP [CaMKII] - Camui [CaMKII] - FRESCA [CaMKII] - K2alpha [cAMP] - cADDis [cAMP] - camps [cAMP] - CAMYEL [cAMP] - CUTie [cAMP] - Epac-S [cAMP] - Epac1 (delta DEP-CD) based cAMP biosensors [cAMP] - Flamindo [cAMP] - FPX [cAMP] - ICUE [cAMP] - mCRIS based cAMP biosensor [cAMP] - mICNBD [cAMP] - mICNBD-FRET [cAMP] - Nano-lantern cAMP [cAMP] - R-FlincA [cAMP] - R1alpha #7 [cAMP] - Split Luc cAMP biosensor [cAMP] - YFP-PKAc + CFP-PKAr [CaN] - CaN Activation [CaN] - CaNAR [CaN] - CaNARi [CaN] - DuoCaN [Caspase1] - FPX caspase 1 sensor [Caspase1] - SCAT1 [Caspase3] - CASPAM [Caspase3] - ddFP based [Caspase3] - DEVD [Caspase3] - DEVK [Caspase3] - EC-RP [Caspase3] - Far-Red Caspase sensors [Caspase3] - Flip-GFP [Caspase3] - Intein Based [Caspase3] - iProtease [Caspase3] - SCAT3 [Caspase3] - yDMQDc [Caspase8] - Bid cleavage sensor [Caspase8] - FPX caspase 8 sensor [Caspase8] - IC-RP [Caspase9] - SCAT9 [Cd2+] - Cd-FRET [Cdc42] - (CDC42) GEF Sensor [Cdc42] - CDC42 Biosensor [Cdc42] - Cdc42 FRET [Cdc42] - CDC42 Raichu [Cdc42] - Cdc42-2G [Cdc42] - ddFP Cdc42 Biosensor [Cdc42] - GDI Cdc42 FLARE [CDK1] - CyclinB1-Cdk1 activity sensor [CDK2] - CDK2 Translocation Reporter [Cell Cycle Phases] - Fucci [cGMP] - cGES [cGMP] - cGKI based cGMP sensors [cGMP] - cGull [cGMP] - CGY [cGMP] - CUTie [cGMP] - cyGNAL [cGMP] - cygnet [cGMP] - FlincG [cGMP] - PfPKG [Citrate] - FLIP-Cit [CO] - COSer [CRAC] - PH(crac)-GFP [CREB] - ICAP [Cu+] - CreiLOV [Cu+] - Yeast Copper regulator based [DAG] - Cys1-GFP [DAG] - Daglas [DAG] - DAGR [DAG] - Digda [DAPK1] - DAPK1 sensor [Dictyostelium GPCR] - Labeled G proteins [DNA methylation] - DNA methylation BiAD [Dopamine] - dLight : Dopamine [Dopamine] - Dopamine CNiFER [Dopamine] - GRAB-DA [DRD2] - iTango [eCB] - GRAB-eCB [EGFR] - ECaus [EGFR] - EGFR Reporter [EGFR] - FLAME [EGFR] - Picchu-Z [Enterokinase] - BRET-based [ERK] - EAS [ERK] - EKAR [ERK] - ERK-KTR [ERK] - Erkus [ERK] - ERKy [ERK] - FIRE [ERK] - FLINC-EKAR1 [ERK] - FPX ERK [ERK] - Miu2 [F-1,6-BP] - fructose‐1,6‐bisphosphate [FAK] - FAK Activation Biosensor [FAK] - FAK Autophosphorylation Biosensor [FAK] - FAK sensor [Fus3] - Far1-SKARS [Fyn] - FRET [Fyn] - saFRET [FZD4] - Receptor Conformation Sensor [FZD5] - Receptor Conformation Sensor [FZD6] - Receptor Conformation Sensor [FZD7] - Receptor Conformation Sensor [G1 Phase] - G1 Phase Biosensor [GABA] - GABA-Snifit [GABA] - iGABASnFR [GCK] - GCK Activation Biosensors [Gα] - Galpha activation [Gαi] - Gαi Sensor [Gαq] - Gαq Sensor [Gαs] - Gαs Sensor [Gαs] - Gs Activation BRET Assay [Gαs] - Nb37 based [Gln] - FLIPQ [Glucose] - FLIPglu [Glucose] - GGBP [Glucose] - GIP [Glucose] - Glifon [Glutamate] - FLIP Glt [Glutamate] - FLIPE [Glutamate] - GluSnFR [Glutamate] - iGluSnFR [Glutamate] - QBP based Gln Sensor [Glutamate] - Snifit-iGluR5 [Glycine] - glycine FRET sensor [GTP] - GEVAL [H2O2] - HyPer [H2O2] - roGFP based H2O2 sensor [H2O2] - Unnatural Amino Acid H2O2 sensor [H2O2] - Yap1 based H2O2 sensor [H2S] - pAzF H2S sensors [H3-S10p] - H3S10p biosensor [H3-S28p] - Histone Phosphorylation reporter [H3K9me3] - H3K9me3 BiAD [H3R] - Receptor Conformation Sensor [Halide Ions] - Clomeleon [Halide Ions] - Dual pH and Cl sensor [Halide Ions] - Halide Sensitive Fluorescent Proteins [Heme] - CHY [Heme] - CISDY [Heme] - Fluorescence quenching Heme [Heterotrimeric G Proteins] - αβγ trimerization [Hg(II)] - eGFP based Hg sensor [Hg(II)] - IFP based Hg sensor [His] - FHisJ [His] - FLIP-HisJ [Histone Acetylation] - Histac [Ins(1,3,4,5)P4] - Btk PH domain based [INSR] - Insulin Receptor Activation BRET assay [INSR] - Phocus [INSR] - sinphos [Insulin] - RINS [IP3] - FIRE [IP3] - fretino [IP3] - GFP-PH [IP3] - IRIS [IP3] - LIBRA [α1-AR] - α1-AR SPASM [α2A-AR] - dLight [α2A-AR] - α2-AR SPASM [α2A-AR] - α2A-AR + labeled G protein [α2A-AR] - α2A-AR activation sensor [JNK] - dJUN-FRET [JNK] - JNK-KTR [JNK] - JNKAR [JNK] - JuCKY [K+] - GEPII [K+] - GINKO [K27H3 methlytransferase] - K27 Reporter [K9H3 methyltransferase] - K9 Reporter [kappa-opioid receptor] - dLight [Kss1] - Ste7-SKARS [Lactate] - Laconic [LATS] - LATS-BS [LCACoAs] - LACSer sensor [Lck] - Lck Activation sensor [Ln3+] - LaMP [M1R] - M1R Activation Sensor [M3R] - Receptor Conformation Sensor [malonyl-CoA] - FapR-NLuc [maltose] - FLIPmal [maltose] - MBP [MAPK or MK2] - GMB [MARK] - MARK sensor [Matriptase] - ddFP-based [Mechanical Strain] - HP35 [Mechanical Strain] - PriSSM [Mechanical Strain] - stFRET [Mechanical Strain] - TSMod [Mg2+] - MagFRET [Mg2+] - MagIC [MLKL] - SMART [Mn2+] - MnLaMP [Molecular crowding] - CrGE [Molecular crowding] - Crowding sensor [Mpk1] - Mkk2-SKARS [Mpro] - BRET-based [Mpro] - Flip-GFP [MT1-MMP] - MT1-MMP Biosensor [MT2] - dLight [mTORC1] - TORCAR [mu-opioid receptor] - dLight [N-WASP] - N-WASP BS [NAD+] - NAD+ Biosensor [NAD+] - NAD-Snifit [NAD+/NADH Ratio] - Peredox [NAD+/NADH Ratio] - SoNar [NAD+/NADH Ratio] - T-Rex Based [NADH] - Frex [NADP+] - Apollo-NADP+ [NADP+] - NADPsor [NADP+:NADPH Ratio] - NADP-Snifit [NADPH] - iNap [NADPH] - Semi-synthetic BRET Sensor [NE] - NE CNiFER [Nicotine] - iNicSnFR [NO] - FRET-MT [NO] - geNOps [NO] - sGC based NO biosensor [O2] - dUnOHR hypoxia-reoxygenation sensor [O2] - FluBO [ODR-10] - OGOR [OGT] - O-GlcNAc Sensor [OHP] - OHSer [ONOO-] - pnGFP [opsin] - opsin SPASM [Orexin Neuropeptide] - dLight : Orexin [Oxytocin] - GRAB-OT [p38] - p38 activity reporter [p38] - p38-KTR [PA] - Pii [PAK1] - Pakabi [PDGFR] - PDGFR Biosensor [PDK1] - PARE [pH] - deGFP [pH] - Dendra [pH] - Dual pH and Cl sensor [pH] - E^xGFP [pH] - FluBpH [pH] - mtAlpHi [pH] - Native FP pH Sensitivity [pH] - pHaROS [pH] - pHCEC [pH] - pHluorins [pH] - pHRed [pH] - pHTomato [pH] - pHuji [pH] - pHusion [pH] - SRpHi [pH] - SypHer [pH] - XFpH [Phosphate] - FLIPPi [PI(3)P] - GFP-FYVE [PI(3)P] - GFP-PX [PI(3,4)P2] - cPHx3 [PI(3,4)P2] - Pippi-PI(3,4)P2 [PI(3,5)P2] - GFP-MLN1 [PI(4)P] - BRET PI(4)P sensors [PI(4)P] - Pippi [PI(4,5)P2] - CAY [PI(4,5)P2] - CYPHR [PI(4,5)P2] - ENTH-GFP [PI(4,5)P2] - FP-Tubby [PI(4,5)P2] - FPX PIP2 sensor [PI(4,5)P2] - PH(PLCdelta) [PI(4,5)P2] - Pippi-PI(4,5)P2 [PI(4,5)P2] - R-PRE [PIP3] - FLLIP [PIP3] - InPAkt [PIP3] - Labeled PH domains [PIP3] - PIP3 BRET sensor [PKA] - AKAR [PKA] - ART [PKA] - FLINC-AKAR1 [PKA] - PKA-KTR [PKA] - RLuc-PCA PKA [PKA] - Single Color PKA sensor [PKC] - CKAR [PKC] - Eevee-PKC [PKC] - IDOCKS [PKC] - ITRACK [PKC] - KCP [PKD] - DKAR [PKD] - G-PKDrep [PKM2] - PKAR [PMCA] - PMCA Activity Sensor [Pn] - EcPhnD based [PS] - 2xPH(EVECTIN2) [PS] - cPLA2-C2 [PS] - Lact-C2 [PS] - PKC-C2 [PS] - PLCdelta1-C2 [PTEN] - Rluc-PTEN-YFP [PTH1R] - Receptor Conformation Sensor [PTHR] - PTHR activation sensor [Pyruvate] - PdhR Based [Rab13] - Rab13 [Rab5] - Rab5 Raichu [Rac1] - ddFP Rac1 sensors [Rac1] - FLAIR [Rac1] - FLARE [Rac1] - GDI Rac1 FLARE [Rac1] - Rac1 Raichu [Rac1] - Single Chain Rac1 biosensors [Rac2] - Single Chain Rac2 biosensors [Rac3] - Single Chain Rac3 biosensors [Ral] - Raichu-Ral [Ran] - Ran FRET probes [Rap1 ] - Rap1 Raichu [Rap1 ] - Rap1-FLARE [Ras] - ddFP Ras Biosensor [Ras] - DORA Ras [Ras] - FRas [Ras] - Ras Raichu [Ras] - RasAR [redox status] - HSP33 [redox status] - redox-sensitive linker [redox status] - roFP [redox status] - rxRFP [redox status] - rxYFP [Rho] - GBD [RhoA] - GDI-RhoA FLARE [RhoA] - RhoA FLARE [RhoA] - RhoA FRET [RhoA] - RhoA Raichu [RhoB] - DORA RhoB [RhoC] - DORA RhoC [RhoC] - RhoC FLARE [RhoQ] - TC10 Raichu [ribose] - FLIPrib [ROCK] - Eevee-ROCK [ROS] - pHaROS [ROS] - pHaROS [RRas] - RRas Raichu [RSK] - Eevee-RSK [RTK] - Picchu [S Phase] - S Phase Biosensor [S6K] - Eevee-S6K [SAP3K] - SAP3K [SnRK2.6/OST1] - SNACS [Src] - Src Indicator [Src] - Srcus [sterol] - mCherry-D4H [sucrose] - FLIPsuc [superoxide] - mt-cpYFP [Temperature] - ELP [Temperature] - gTEMP [Temperature] - mRFP [Temperature] - tsGFP [TEV] - Flip-GFP [Trehalose] - FLIP [TrkB] - TrkB-PLC interaction [Trp] - FLIPW [Trp] - GRIT [Ubiquitination] - REACh-Ubiquitin [V+] - ArcLight Species Variants [V+] - Ci-VSP based sensors [V+] - Danio VSP based sensor [V+] - Kv Based V sensors [V+] - Na Channel based V Sensors [V+] - Nematostella VSP based sensors [V+] - rhodopsin based V sensors [VEGF] - BRET VEGF biosensor [ZAP-70] - ROZA [ZAP-70] - saFRET [Zn2+] - Atox1 WD4 Based Zn2+ Probes [Zn2+] - GZnP [Zn2+] - Minimal Zinc Finger Based Zn Probes [Zn2+] - Zap1 Based Zn Probes [Zn2+] - ZinCh
Notes

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