Daglas-em1

fluorescent indicator for DAG that is locally generated at subcellular membranes

DAG

binding

Unimolecular

FRET

N/A

CFP | rigid linker 1 | Cysteine-rich domain (CRD) from PKCbeta | rigid linker 2 w/hinge | YFP | rigid linker 3 | endomembrane localization sequence

Cysteine-rich domain (CRD) from PKCbeta

CFP | YFP

ER and Golgi