Cal-Light - Ca2+ Fluorescent Biosensor Details

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Biosensor Name (short):

Cal-Light

Long name :

Biosensor Target:

Ca2+

Sensing Type:

binding

Number of Molecules:

Bimolecular or other

Physics Type:

Intensity

Biosensor Group:

Ca2+ Snapshot

Biosensor Sub-group:

N/A

Sensor Components:

TM PM targeting | CaM | TEV-N |AsLOV2|TEVseq|tTA||M13|TEV-C|| TetO|EGFP

Sensing Element:

CaM | M13 | AsLOV2

Source Pubmed ID:

28650460 [Pubmed]

Fluorescent Proteins:

EGFP

Addgene Plasmid #:

Addgene #92390 , Addgene #92389

Subcellular Targeting :

Other Source Pubmed IDs:

Notes:

Integrates cells with high Ca2+ while being illuminated by blue light. The blue light causes AsLOV2 to release the TEV sequence which can be cut by the recombined TEV