FLARE - Ca2+ Fluorescent Biosensor Details

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Biosensor Name (short):

FLARE

Long name :

Biosensor Target:

Ca2+

Sensing Type:

binding

Number of Molecules:

Unimolecular

Physics Type:

Intensity

Biosensor Group:

Ca2+ Snapshot

Biosensor Sub-group:

N/A

Sensor Components:

NRX-TM | CaM bp | eLOV-J alpha | TEV sequence | tTA-VP16 || CaM | TEV (1-219) || Tet-O | GFP

Sensing Element:

CaM bp | eLOV-J alpha | CaM

Source Pubmed ID:

28650461 [Pubmed]

Fluorescent Proteins:

GFP

Addgene Plasmid #:

Addgene #92213 , Addgene #92214 , Addgene #92202

Subcellular Targeting :

Other Source Pubmed IDs:

Notes:

Integrates cells with high Ca2+ while being illuminated by blue light. The blue light causes AsLOV2 to release the TEV sequence which can be cut by the recombined TEV