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FLARE
Ca2+
binding
Unimolecular
Intensity
N/A
NRX-TM | CaM bp | eLOV-J alpha | TEV sequence | tTA-VP16 || CaM | TEV (1-219) || Tet-O | GFP
CaM bp | eLOV-J alpha | CaM
GFP
Integrates cells with high Ca2+ while being illuminated by blue light. The blue light causes AsLOV2 to release the TEV sequence which can be cut by the recombined TEV