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GACh1.0
GPCR-based acetylcholine sensor
ACh
binding
Unimolecular
Intensity
N/A
MR Trans membrane domains 1-5 |GG Linker | cpGFP| GGAAA Linker |MR TM domains 6-7
Muscarinic acetylcholine receptor (MR)
cpGFP
Plasma Membrane
GACh has the sensitivity, specificity, SNR, kinetics and photostability suitable for monitoring ACh signals in vitro and in vivo