NADP-Snifit - NADP+:NADPH Ratio Fluorescent Biosensor Details

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Biosensor Name (short):

NADP-Snifit

Long name :

NADP Semisynthetic fluorescent sensor protein

Biosensor Target:

NADP+:NADPH Ratio

Sensing Type:

binding

Number of Molecules:

Unimolecular

Physics Type:

FRET

Biosensor Group:

NADP-Snifit

Biosensor Sub-group:

N/A

Sensor Components:

Sepiapterin reductase (SPR) | HaloTag labeled with Siliconrhodamine | Proline30 linker | SNAP-Tag labeled with Tetramethylrhodamine-Sulfamethoxazole |

Sensing Element:

Sepiapterin reductase (SPR) | Sulfamethoxazole (SMX)

Source Pubmed ID:

29809136 [Pubmed]

Fluorescent Proteins:

Siliconrhodamine-Halo | Tetramethylrhodamine (CP-TMR-SMX)

Addgene Plasmid #:

Addgene #113917

Subcellular Targeting :

Cytosol, nucleus or mitochondria

Other Source Pubmed IDs:

Notes:

Also created a version with HaloTag replaced with EGFP in the supplement of 29809136