NAD-Snifit - NAD+ Fluorescent Biosensor Details

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Biosensor Name (short):

NAD-Snifit

Long name :

NAD Semisynthetic fluorescent sensor protein

Biosensor Target:

NAD+

Sensing Type:

binding

Number of Molecules:

Unimolecular

Physics Type:

FRET

Biosensor Group:

NAD-Snifit

Biosensor Sub-group:

N/A

Sensor Components:

Sepiapterin reductase (SPR) A41D, R42W | HaloTag labeled with Siliconrhodamine | Proline30 linker | SNAP-Tag labeled with Tetramethylrhodamine-Sulfamethoxazole

Sensing Element:

Sepiapterin reductase (SPR) A41D, R42W | Sulfamethoxazole

Source Pubmed ID:

29809136 [Pubmed]

Fluorescent Proteins:

HaloTag-Siliconrhodamine; SNAP-Tag-Tetramethylrhodamine

Addgene Plasmid #:

Addgene #113914

Subcellular Targeting :

Cytosol, nucleus or mitochondria

Other Source Pubmed IDs:

Notes:

Mutations in SPR converts specificity from NADP to NAD