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GABA-Snifit
GABA
binding
Bimolecular or other
FRET
N/A
SNAP-tag modified with benzylguanine(BG)-Cy5- intramolecular ligand CGP | TR linker | CLIP-tag conjucated with CLIP-Surface547| TR Linker | GABA B Receptor subunit 1a (GB1a)
intramolecular ligand CGP | GABA B Receptor subunit 1a (GB1a)
Cy5 | Surface547
Extracellular
Semisynthetic biosensor. Other synthetic dye FRET pairs tested in Masharina J Am Chem Soc 2012 as well