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AMPfret 2.1
AMPK
binding
Unimolecular
FRET
N/A
alpha 2 AMPK domain | helix | mseCFP delta 11 | beta 2 AMPK domain | cpVenus | gamma 1 AMPK domain
alpha 2 beta 2 gamma 1 AMPK domains
mseCFP delta 11 | cpVenus
Measures AMPK activation by conformational change in AMPK, not for AMPK activity but the on/off of the kinase itself