MagIC - Mg2+ Fluorescent Biosensor Details

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Biosensor Name (short):

MagIC

Long name :

indicator for "Magnesium Imaging in Cell"

Biosensor Target:

Mg2+

Sensing Type:

binding

Number of Molecules:

Unimolecular

Physics Type:

Ratiometric

Biosensor Group:

MagIC

Biosensor Sub-group:

N/A

Sensor Components:

cp173Venus mutant | mCherry

Sensing Element:

(S220E, S31D, Q33E, V89I, Q142M)-cp173Venus

Source Pubmed ID:

26244765 [Pubmed]

Fluorescent Proteins:

cp173Venus mutant, mCherry

Addgene Plasmid #:

Subcellular Targeting :

available (cytosol-nucleus, mitochondria, ER)

Other Source Pubmed IDs:

Notes:

mCherry is used as a Mg2+-insensitive reference. Kd (Mg2+) = 5.1 mM; Kd (Ca2+) = 4.8 mM. Plasmid requests to Prof. Takeharu Nagai (Osaka University, https://www.sanken.osaka-u.ac.jp/labs/bse/)