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REACh-Ubiquitin
Ubiquitination
Bimolecular or other
GFP | PEST (enriched in proline, glutamic acid, serine, and threonine) ubiquitination substrate || REACh2 | ubiquitin
PEST | ubiquitin
GFP || REACh2
REACh2 is a dark FRET acceptor making this a single color donor quenching readout