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KCAP-1
phosphorylation
Unimolecular
EYFP | Pleckstrin PH domain | Pleckstrin PKC substrate| Pleckstrin DEP domain (18 AA truncation| | Kemptide PKA sequence | GFP
Pleckstrin PH domain | Pleckstrin PKC substrate| Pleckstrin DEP domain (18 AA truncation| | Kemptide PKA sequence
EYFP | GFP
Also measures PKA activity, Increase in FRET = PKA phosphorylation, decrease in FRET = PKC phosporylation